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The 2hydroxyl group affects the tertiary structure of RNA. First the conformation of the sugar is different between DNA and RNA. Lesson Explainer Types Of Rna Nagwa The ssRNAP holds a DNA-RNA hybrid of 8bp. . T7 polymerase has been crystallised in several forms and the structures placed in the PDB. The N-terminal domain moves around as the elongation complex forms. These hydrogen bonds are important in the formation of the tertiary structure of an RNA and are not available to DNA. Secondly the 2hydroxyl group provides hydrogen bond donor and acceptor functions for formation of hydrogen bonds. These explain how T7 polymerase binds to DNA and transcribes it.
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